Kinetics of adenovector production by modified CTLs. (A-B) EBV-specific CTLs were sequentially transduced with both the retrovirus and the Ad5F35-eGFP. After washing, the cells were incubated with (♦) or without (▪) irradiated autologous LCLs for the entire culture period (A) or for 12 hours. (B) Supernatants were collected at different time points and used to transduce A459 cells. The latter were then analyzed by flow cytometry. Results are expressed as the percentage of eGFP-positive A549 cells after 24 hours of exposition to CTL supernatants. The arrows represent the times of stimulation with the irradiated LCLs. (C) The C-RE-AG (Ad5F35-eGFP–transduced CTLs, 40% efficiency) were activated with irradiated LCLs (▴) or CD3/CD28 MAb (▪). Nonactivated (NA) cells were used as negative control (♦). Error bars indicate mean ± SD.