Representative FACS profile of Notch4-IC–transduced cord cells. FACS profile demonstrates that Notch4-IC–transduced cord cells give rise to a CD4⁺CD8⁺ population in the bone marrow of β₂-microglobulin NOD/SCID mice. Marrow from week-9 and week-12 β₂-microglobulin NOD/SCID mice receiving transplants of MIG- and Notch4-IC (n = 4)–transduced cord blood cells was analyzed for phenotype by FACS. Murine marrow cells were stained with CD4-PE and CD8–allophycocyanin (CD8-APC) along with propidium iodide (PI). (A) Marrow from MIG mouse within PI gates. (B) Marrow from Notch4-IC mouse within PI gates.