Multiple alignment of KLRL1 with closely related KLR family receptors. Alignment was performed with the GCG package and minimally adjusted manually. Identical residues are boxed in black, and similar residues are in gray. (A) Alignment of human and mouse KLRL1. Approximate domain boundaries are indicated for the cytoplasmic, transmembrane, stalk, and C-type lectinlike domains (CTLDs). Putative N-glycosylation sites are shown by boxes. (B) Multiple alignment of CTLDs of KLRL1 and closely related KLR family members. Asterisks indicate positions of the conserved invariant residues characteristic of CTLDs. (C) Dendrogram displaying total amino acid identity among some KLR family members. Human, mouse, and rat KLRL1 constitute a separate branch of the KLRs, most closely related to CLEC2 and CLEC1. The GenBank accession numbers of the analyzed sequences are AF247788 (hKLRL1), NM_177686(mKLRL1), XM_232420 (rKLRL1), NM_016509 (hCLEC2), AF201457 (mCLEC2), AF200949 (hCLEC1), AF023840 (hNKG2A), AJ001684 (hNKG2C), AY100458 (mKLRE1), AF486186 (rKLRE1), U30610 (hCD94), AF030311 (mCD94), NM_005810 (hKLRG1), NM_016970 (mKLRG1), NM016523 (hKLRF1), NM_002258 (hKLRB1), AF133299 (hLLT1), and AF416564 (rKLRH1).