KLRL1/partner heterodimer associates with SHP-1 and SHP-2. (A) NK-92 and U937 cells were pretreated with pervanadate (+) or not (-), then digitonin lysates of the cells were incubated with HK13 and protein A beads, and precipitates were subjected to Western blot analysis with anti-SHP-1 and anti-SHP-2. (B) hKLRL1 forms a functional heterodimer with a putative partner molecule. Pervanadate stimulated (5 minutes at 37°C) (+) and unstimulated (-) NK-92 and U937 cells were lysed in 1% digitonin buffer and tested by Western blotting, using anti-hKLRL1 mAb HK13. (C) L929 cells transiently transfected with mouse KLRL1/Flag expression vector were pretreated with pervanadate (+) or untreated (-), then digitonin lysates of the cells were incubated with anti-Flag M2-agarose beads, and precipitates were subjected to Western blot analysis with anti-HP-1, anti-SHP-2, and anti-Flag antibodies. Crude lysates (right hand panels) served as a control. The data shown are representative of 3 independent experiments.