Point mutation in and molecular modeling of G6PD Zurich. (A) Point mutation in G6PD Zurich that leads to alternative splicing and a 9-base deletion. In the cDNA, the exon to the left of the vertical line is exon 10. Exon 11 is shown on the right side. (B) Molecular modeling of G6PD Zurich. G6PD wild type (WT) versus Zurich: an overlap of the wild type (blue and gray) and Zurich mutant (red and orange) structures reveals a change in a loop and an adjacent helix forming part of the substrate binding site. The active Lys205 (shown in CPK) is in direct proximity. The mutation affects helix of the substrate binding site in both monomers (arrows) but only minimally affects the dimer interface. The figures were created with VMD software8 (http://www.ks.uiuc.edu/Research/vmd) and rendered with Povray (http://www.povray.org).
