PMN ectosomes are not proinflammatory but anti-inflammatory. (A) HMDMs were incubated with medium alone (Ø), with medium supplemented with ectosomes, zymosan, or LPS for 24 hours. Supernatants were analyzed for IL-8, IL-10, TNFα, and TGFβ1. (B) HMDMs were incubated with medium alone (Ø), with medium supplemented with zymosan or LPS, with or without ectosomes for 24 hours. Supernatants were analyzed for IL-8, IL-10, TNFα, and TGFβ1. (C) HMDMs were incubated with medium alone (Ø), with medium supplemented with zymosan with or without ectosomes for 24 hours. Supernatants were analyzed for IL-8, IL-10, TNFα, and TGFβ1. Error bar indicates standard deviation of the mean.