Figure 6.
Effect of DTT on KCC activation by cell swelling in SS RBCs. (A) Rb influx activated by pH in SS RBCs with (•) and without (♦) preincubation with DTT (10 mM). DTT was present at 1 mM during nystatin treatment and Rb influx measurement. Data represent 4 independent experiments. (B) Normalized fluxes using maximal volume-stimulated flux (MCHC < 270 g/L [27 g/dL]) of control or DTT-treated SS RBCs. (C) Regulatory volume decrease in SS reticulocytes induced by cell swelling (to MCHC of 260 g/L [26 g/dL]) with and without DTT (n = 4). Error bars indicate standard deviation.