Figure 3.
![Figure 3. DC need less signaling time than B cells to fully activate T cells. DO11.10 T cells (with or without CFSE) were mixed with OVA-loaded B cells or DCs within liquid cultures in round-bottom multiwell plates. At different time points a cocktail of antibodies that inhibited further APC signaling was added to individual wells. (A) After 48 hours, cultures were pulsed with 1 μCi (0.037 MBq) [3H]-thymidine for 12 hours. Subsequently, cell-bound radioactivity was assessed by liquid scintillation counting. Data are means of duplicate measurements for each time point. (B) T-cell proliferation after 48 hours was assessed by FACS, measuring down-regulation of the CFSE label.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/9/10.1182_blood-2004-03-1193/6/zh80210468430003.jpeg?Expires=1735527151&Signature=gOgFtE~HY4tRz2sDqTiHWcfWm1YNNpehwO5J6tE~P3NUhjKE4HAbG2VybEEx5ne0lqh00TZxReIJTMf-CCatJkoUb9HurIaWtekrhKsf8L6o3o~eAcDCvVSaLFwVkPRGuZkYQ~GQFdhOzG886R~D2IqRAem6GgNknuDPrSxIJUcBPsVF7YRwjLE5ItdUZ42KbSMeJMMvg5BfnGGo2G~sMQuL55iBSuhOK6FGmAfNXmuIxlzZUVdqyTnQcVg03D9F4SlMcaixasxNaAvBQnP6ns-0PT~LG4HwGPbbIumS4cJy1dE8YiGw9B2BcZhe3aRI-eLbqH9lBiQSGl4ZRpAB7w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
DC need less signaling time than B cells to fully activate T cells. DO11.10 T cells (with or without CFSE) were mixed with OVA-loaded B cells or DCs within liquid cultures in round-bottom multiwell plates. At different time points a cocktail of antibodies that inhibited further APC signaling was added to individual wells. (A) After 48 hours, cultures were pulsed with 1 μCi (0.037 MBq) [3H]-thymidine for 12 hours. Subsequently, cell-bound radioactivity was assessed by liquid scintillation counting. Data are means of duplicate measurements for each time point. (B) T-cell proliferation after 48 hours was assessed by FACS, measuring down-regulation of the CFSE label.
