Figure 3.
![Figure 3. Antigen but not KL induces translocation of PKC. Human mast cells were incubated overnight in the presence of IgE and in the absence of KL and IL-6, as described in the legend for Figure 1, and then either not stimulated or stimulated with 100 ng/mL antigen (Ag), KL, or both stimulants for 5 minutes. Immunoblots were prepared from the membrane fraction of whole-cell lysates using antibodies against the indicated isoforms of PKC, phosphorylated PKCα/β [p-PKC (α+β)], or phosphorylated pan-PKC [p-PKC (pan)]. The photographs were overexposed to maximize detection of membrane bound PKC in KL-stimulated cells. Identical results were obtained in 2 other experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/8/10.1182_blood-2004-02-0631/6/zh80200467680003.jpeg?Expires=1735830881&Signature=ux4I5JyPL-2TNV1iEVnqZVBLRDkJwpDkI6hni3s9yoQf6fjPjd03XEq6I6KdR-0s3r6uF3~09CKXGq83uVAVaDGBvxyRH1K78duI4PbLX~2tYMipSHLT7BcC2wNM1EHVkiSlsLGathvqvWMFakHNY8yvVPHbqubNz4PJIbGyv9iP~n7FTy8rSIj~htkisHB70~2djKS2CQGBLOzVerOIwgwphcyJUaGxF3hwj2CZLiTvvWcJZ8dDLsMzLEGJjZYVcTgrw9ueOgD7cR4x~rUgxyo3rvYfX31O-l2smtvA8kGxY39JGpMlwuBxHF-mqscuYCum7H0-2gFCnRyaGn1iNQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Antigen but not KL induces translocation of PKC. Human mast cells were incubated overnight in the presence of IgE and in the absence of KL and IL-6, as described in the legend for Figure 1, and then either not stimulated or stimulated with 100 ng/mL antigen (Ag), KL, or both stimulants for 5 minutes. Immunoblots were prepared from the membrane fraction of whole-cell lysates using antibodies against the indicated isoforms of PKC, phosphorylated PKCα/β [p-PKC (α+β)], or phosphorylated pan-PKC [p-PKC (pan)]. The photographs were overexposed to maximize detection of membrane bound PKC in KL-stimulated cells. Identical results were obtained in 2 other experiments.
