Figure 2.
Figure 2. Phenotypic and functional analysis of adult and cord blood EPC-derived endothelial cells. (A-B) Immunophenotyping of cell monolayers derived from adult peripheral blood (AB; panel A) or umbilical cord blood (CB; panel B) EPC-derived ECCs by fluorescence cytometry. Cord blood and adult EPC-derived ECCs express CD31, CD141, CD105, CD146, CD144, VWF, and Flk-1 but do not express CD45 and CD14. Some cord blood and adult cells express CD34, CD133, and CD117. Shown are representative data from 18 independent experiments using different adult cell monolayers and 13 independent experiments using different cord blood cell monolayers with similar results. Isotype controls are overlaid in a gray line on each histogram for each surface antigen tested. (C) Adult and cord blood EPC-derived endothelial cells incorporate DiI-Ac-LDL (50 × magnification). A representative photomicrograph is shown for adult and cord blood EPC-derived endothelial cells, which have taken up DiI-Ac-LDL (red) and have also stained with DAPI (blue). Shown are representative data from 18 independent experiments using different adult cell monolayers and 13 independent experiments using different cord blood cell monolayers with similar results. Scale bar represents 100 μm. (D) Adult and cord blood EPC-derived endothelial cells expressing EGFP were plated in Matrigel for the formation of capillary-like structures (50 × magnification). Shown are representative data from 18 independent experiments using different adult cell monolayers and 13 independent experiments using different cord blood cell monolayers with similar results. Scale bar represents 100 μm. (E) Adult and cord blood EPC-derived endothelial cells up-regulate the cell-surface expression of VCAM-1 in response to either rhTNF-α or rhIL-1. Shown are representative data from 18 independent experiments using different adult cell monolayers and 13 independent experiments using different cord blood cell monolayers with similar results. The isotype control for VCAM-1 is overlaid in a gray line on each histogram.

Phenotypic and functional analysis of adult and cord blood EPC-derived endothelial cells. (A-B) Immunophenotyping of cell monolayers derived from adult peripheral blood (AB; panel A) or umbilical cord blood (CB; panel B) EPC-derived ECCs by fluorescence cytometry. Cord blood and adult EPC-derived ECCs express CD31, CD141, CD105, CD146, CD144, VWF, and Flk-1 but do not express CD45 and CD14. Some cord blood and adult cells express CD34, CD133, and CD117. Shown are representative data from 18 independent experiments using different adult cell monolayers and 13 independent experiments using different cord blood cell monolayers with similar results. Isotype controls are overlaid in a gray line on each histogram for each surface antigen tested. (C) Adult and cord blood EPC-derived endothelial cells incorporate DiI-Ac-LDL (50 × magnification). A representative photomicrograph is shown for adult and cord blood EPC-derived endothelial cells, which have taken up DiI-Ac-LDL (red) and have also stained with DAPI (blue). Shown are representative data from 18 independent experiments using different adult cell monolayers and 13 independent experiments using different cord blood cell monolayers with similar results. Scale bar represents 100 μm. (D) Adult and cord blood EPC-derived endothelial cells expressing EGFP were plated in Matrigel for the formation of capillary-like structures (50 × magnification). Shown are representative data from 18 independent experiments using different adult cell monolayers and 13 independent experiments using different cord blood cell monolayers with similar results. Scale bar represents 100 μm. (E) Adult and cord blood EPC-derived endothelial cells up-regulate the cell-surface expression of VCAM-1 in response to either rhTNF-α or rhIL-1. Shown are representative data from 18 independent experiments using different adult cell monolayers and 13 independent experiments using different cord blood cell monolayers with similar results. The isotype control for VCAM-1 is overlaid in a gray line on each histogram.

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