Rescue of TF and fibrin accumulation in LTF mice that received bone marrow transplants from wild type mice (WT/LTF chimeras). (A) TF, fibrin, and platelet accumulation in thrombi of wild-type mice that received bone marrow transplants from wild type mice (WT/WT) and LTF mice that received bone marrow transplants from wild type mice (WT/LTF chimeric mice). The thrombus is shown at varying stages of its development as described in Figure 2A. Top panels, WT/WT; bottom panels, WT/LTF chimeric mice. Intravital 4-channel images are as described in Figure 2A. In all experiments, infusion of anti-CD41 was used to detect platelets, antifibrin-specific antibodies were used to detect fibrin, and anti-TF antibodies used to detect TF antigen. (B) Platelet accumulation in thrombi formed in WT/WT mice, WT/LTF chimeric mice, and low TF mice. Median values of integrated platelet fluorescence intensity (F_P) are shown for 43 thrombi from 4 WT/WT mice, 15 thrombi from 3 low TF mice, and 25 thrombi from 3 WT/LTF chimeric mice. (C) Ratio of integrated fluorescence intensity associated with TF to the integrated fluorescence intensity associated with platelets in WT/WT mice, low TF mice, and WT/LTF chimeric mice. All values shown are median values. WT/WT, 43 thrombi in 4 mice; low TF, 15 thrombi in 3 mice; WT/LTF, 25 thrombi in 3 mice. Left panel, early phase; right panel, plateau phase. (D) Ratio of integrated fluorescence intensity associated with fibrin to the integrated fluorescence intensity associated with platelets in WT/WT mice, low TF mice, and WT/LTF chimeric mice. All values shown are median values. WT/WT, 43 thrombi in 4 mice; low TF mice, 15 thrombi in 3 mice; WT/LTF chimeras, 25 thrombi in 3 mice. Left panel, early phase; right panel, plateau phase. ^*P < .05; ^**P < .025; ^***P < .0025; ^****P < .0005.