Up-regulation of Ndrg1 mRNA levels after Fe chelation with DFO and 311 is rapid but is not induced by the DNA-damaging agent Act D. (A) MCF-7 cells were incubated with DFO (250 μM), 311 (25 μM), or Act D (5 nM) for 3 to 24 hours at 37°C. (B) The expression of Ndrg1 and TfR1 mRNA was then assessed by semiquantitative RT-PCR (described in “Materials and methods”). Densitometry was performed, and gene expression was then calculated relative to the β-actin control. Results show a typical experiment from 3 performed.