Ndrg1 protein levels were up-regulated after Fe chelation with DFO and 311 but was not induced by the DNA-damaging agent Act D. (A) MCF-7 cells were incubated with DFO (250 μM), 311 (25 μM), or Act D (5 nM) for 3 to 24 hours at 37°C. Protein was then isolated and Western blot analysis was performed (see “Materials and methods” for details). (B) Densitometry was performed, and protein expression was then calculated relative to the β-actin control. Results show a typical experiment from 3 performed.