Figure 3.
PARP cleavage occurs following treatment of BLIN-3 and BLIN-4L with C9i. BLIN-3 and BLIN-4L cells were removed from fibroblast monolayers and cultured for 18 hours in DMSO (D), 50 μM C9i, or 50 μM PCi. Cells were then lysed and analyzed by Western blotting. BID was used as loading control. Lanes labeled 0 h represent lysates from freshly isolated cells. The percentage of annexin V+ cells is shown for each condition.