Figure 4.
BLIN leukemic cells are sensitive to C9i independent of culture conditions. BLIN-1, BLIN-3, and BLIN-4L were plated in medium alone, in medium supplemented with 10 ng/mL IL-7, on adherent fibroblasts, or on adherent fibroblasts supplemented with 10 ng/mL IL-7. All wells had either DMSO, 50 μM C9i, or 50 μM PCi. Following 18-hour incubation, the wells were harvested and the cells were stained with annexin V and analyzed by flow cytometry. Annexin V percentages include PI+ and PI- events. Each bar represents the mean of duplicate values.