Effects of PKG and PKA inhibitors on the NO-mediated inhibition of the thrombin-induced shape change. Aspirin-treated and gel-filtered human platelets in the presence of CP/CPK were stimulated with thrombin (0.01 U/mL) and NO (1.1 × 10^-10 mol NO/mL/min) after 30 minutes of preincubation with vehicle (water; A), 0.5 mM Rp-8-pCPT-cGMPS (B), or 0.5 mM Rp-8-Br-cAMPS plus 0.5 mM Rp-cAMPS (C). Traces are from a single experiment, representative of 3. The experimental conditions are as explained in the legend to Figure 1.