Figure 4.
Chondrogenic potential of CR and BM cells. Passage 2 cells were plated at 50 cells/cm2, cultured for 14 days, and then harvested. The cells were pelleted and cultured in chondrogenesis medium for 21 days. (A) Representative macro picture. Scale bar = 1 mm. (B) Histology section stained with Toluidine Blue. Original magnification, × 100. (C) Immunohistochemistry for type II collagen. Original magnification: × 100 (B-C). (D) The cartilage pellets derived from CR cells and BM cells collected from the 5 young and 3 elderly donors were weighed and the data expressed as mean ± SD (n = 3).
