Gene expression profile of CR cells and BM cells. RNA was prepared from CR cells just after collagenase digestion, 14 days after plating Passage 0 CR cells, and 14 days after plating Passage 0 BM cells. (A) Reverse transcription-PCR analysis. CBFA1 indicates core binding factor alpha 1; OSX, Osterix; PPARγ, peroxisome proliferator activated receptor γ; and FABP4, fatty acid binding protein 4. (B-C) Quantification analysis used by real-time PCR analysis for CBFA-1 (B) and PPARγ (C). β-actin was used as an internal control. The data are expressed as mean ± SD (n = 3). ^*P < .01; n.s. indicates not significant.