Figure 3.
Proliferative potential of CR cells and BM cells. (A) CR cells and BM cells at Passage 1 from 13 donors were plated at 50 cells/cm2 and cultured for 14 days. Average fold increase (n = 3) was calculated and plotted. (B-C) Passage 1 CR cells and BM cells from 6 young donors and 3 elderly donors were plated at 100 cells per 60-cm2 dish in 6 dishes and cultured for 14 days. Then, 3 dishes from each donor were stained with Crystal Violet to count colony number. (B) Colony-forming efficiency is shown. (C) Total cell number was counted from 3 other dishes and cell number per colony was calculated. The data are expressed as mean ± SD. *P < .01
