Figure 1.
![Figure 1. GPI-anchor-deficient phenotype of alveolar macrophages and dendritic cells. (A) Alveolar macrophages (AM; left) or dendritic cells (DC; middle) cultured for 6 weeks were stained with mAb anti-HSA. Peripheral blood leukocytes gated for lymphocytes (PBL) were stained with mAb anti-CD48 (right), which is expressed on both T and B lymphocytes. Shown are fluorescence intensity plots of cells from littermate control (thin dotted lines) or LysMCre/Pig-aflox mice (bold lines), and antibody controls (dashed lines). (B-C) Responses to LPS. Control (wild type [WT]; •) or GPI-anchor-deficient (knock out [KO]; ○) AMs (B) or DCs (C) were stimulated with LPS; mean concentrations of TNF-α released of at least 3 independent experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/9/10.1182_blood-2004-02-0671/6/zh80210468490001.jpeg?Expires=1735548867&Signature=zqsUtcLVOb8TL9b9kWzSSBJJDCvNcCBf3VvHyLF3Z88ikBTstK7PIzlal3OS3sYLVm3VMQKUarJn7BhpV4aEdNQ1ESsOimqYD59TWiVfviGFAFSwCwU39Ys8t4iuYeJW~PQIIy~wK6OHq-MhcU~ovdv72R3zb84CEGPMGXtsfbBlxIDJ6WaR--DhtutYizikf1dEGnvtYcYVZpxlwYA0fC9z4NDrzgw0aODUR-W5vppfJAJ6O8LJClWcSiihbW8NHQEWpqmBrvhbHJ~P759-eUWXZcbyz-oiALJa6Wy3F-UFbSfC9aWdce8wZFKVo1BsnwayQMStRBFuqTMdTQ81tA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
GPI-anchor-deficient phenotype of alveolar macrophages and dendritic cells. (A) Alveolar macrophages (AM; left) or dendritic cells (DC; middle) cultured for 6 weeks were stained with mAb anti-HSA. Peripheral blood leukocytes gated for lymphocytes (PBL) were stained with mAb anti-CD48 (right), which is expressed on both T and B lymphocytes. Shown are fluorescence intensity plots of cells from littermate control (thin dotted lines) or LysMCre/Pig-aflox mice (bold lines), and antibody controls (dashed lines). (B-C) Responses to LPS. Control (wild type [WT]; •) or GPI-anchor-deficient (knock out [KO]; ○) AMs (B) or DCs (C) were stimulated with LPS; mean concentrations of TNF-α released of at least 3 independent experiments are shown.
