Attenuated IgG-IC-induced alveolar inflammation. Mice were injected intravenously with OVA, and intratracheally (i.t.) with either rabbit IgG anti-OVA or saline as control. Then, 4 hours later, the bronchoalveolar (BAL) fluids were isolated for further analysis. (A) The cellular content of the BAL fluids was examined by microscopy. (Left) Control mice injected intratracheally with saline. (Middle) Control mice injected intratracheally with IgG anti-OVA. (Right) LysMCre/Pig-a^flox mice injected intratracheally with IgG anti-OVA. Concentrations of (B) TNF-α and (C) IL-6, and numbers of (D) inflammatory neutrophils (PMN) and (E) red blood cells (RBC) in the BAL fluids were determined. (B-D) Shown are mean values ± SEM of at least 3 independent experiments in control mice (WT; ▪) or LysMCre/Pig-a^flox mice (KO; ▦). ^*Significant differences between WT and KO mice (P < .05).