Down-regulation of Mcl-1 by Mcl-1 siRNA induces apoptosis in MM cells. (A) Mcl-1 down-regulation by Mcl-1 siRNA induces cytotoxicity. MM1s cells (2 × 104 cells per well) were transfected with Mcl-1 siRNA or scrambled siRNA (5 μg), followed by culture for 24 and 48 hours in 96-well plates. MM1s cell survival was determined at 24 and 48 hours by MTT assay. Results shown are percentage of viability compared with control. Error bars indicate standard deviation. (B) Mcl-1 down-regulation by Mcl-1 siRNA induces apoptosis. Cell cycle analysis was performed at 24 and 48 hours (top row and bottom row, respectively) following transfection with Mcl-1 siRNA or scrambled siRNA (5 μg) (right column and left column, respectively). A sub-G1 peak represents apoptotic cells. Shown is 1 representative experiment of 3.
