Figure 2.
Concentration- and time-dependent release of histamine. (A) PBMCs were incubated with increasing concentrations of anti-LIR7 (m473) (▨), with an optimal concentration of anti-FcϵRI (0.1 μg/mL) (▦), or with control IgG1 (□), and then stimulated by addition of goat anti-mouse IgG for 45 minutes (n = 3). (B) PBMCs were incubated with 0.1 μg/mL anti-FcϵRI (•), anti-LIR7 (▪), or control IgG1 (▴) and then stimulated by addition of goat anti-mouse IgG for the indicated times (n = 4).