American Society of Hematology

Figure 2.

$Figure 2. Proliferation enhances FucT VII and C2 GlcNAcT mRNA expression in activated CD4+ lymphocytes. On day 3 after activation as in Figure 1, transgenic KJ1-26.1+ T cells were sorted according to the CFSE stain into individual generations (G0 to G3) (A). FucT VII mRNA and C2 GlcNAcT mRNA expression in relation to HPRT as a housekeeping gene was determined by quantitative PCR within these sorted fractions and in naive T cells (B-C). One representative of 2 experiments is shown.$

Proliferation enhances FucT VII and C2 GlcNAcT mRNA expression in activated CD4⁺ lymphocytes. On day 3 after activation as in Figure 1, transgenic KJ1-26.1⁺ T cells were sorted according to the CFSE stain into individual generations (G0 to G3) (A). FucT VII mRNA and C2 GlcNAcT mRNA expression in relation to HPRT as a housekeeping gene was determined by quantitative PCR within these sorted fractions and in naive T cells (B-C). One representative of 2 experiments is shown.

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