Figure 4.
Cell-cycle arrest prevents up-regulation of FucT VII and C2 GlcNAcT mRNA expression. A total of 1 × 106 naive T cells were activated by plate-bound anti-CD3 and soluble anti-CD28 plus IL-12, IFN-γ, and anti-IL-4 for 3 days. The relative expression of FucT VII (A) and C2 GlcNAcT (B) mRNA to housekeeping gene HPRT was determined for naive T cells and cells activated in the presence or absence of l-mimosine. The mean (+ SD) from 4 individual experiments is shown.
