Adhesion molecules expressed on WT and IFN-γ knockout tumors. Fresh tumor cell suspensions from 4 tumor specimens from each genotype were stained with FITC-conjugated antibodies at a concentration of 1 μg/106 cells and subjected to FACS analysis. (A) FACS scans of IL-2Rβ- and ICAM-1-expressing tumor cells corresponding to one representative tumor and quantitative analysis of the percentage of positive cells in the population from 4 tumor specimens of each genotype are depicted. (B) Representative FACS histograms are shown, and quantitative mean fluorescence intensity comparisons of MHC I and MHC II stains on tumor cells are shown. There were 25 000 tumor cells counted from each tumor, and 4 tumors were analyzed for each genotype. The first peak in all 4 panels indicates isotype control. The second peak corresponds to specific staining for IL-2Rβ, ICAM-1, MHC-I, or MHC-II. Error bars represent SEM.
