Effect of AdDNp67 on TNF-induced intercellular gap formation VE-cadherin distribution. Live, unfixed HUVECs (original magnification, × 100) were coinfected with Ad-VE-cadherin GFP (A-C) and either Ad-lacZ (A-B) or Ad-DNp67 (C). Monolayers exposed to vehicle (A) maintained cell-cell contacts, and Ad-VE-cadherin GFP was localized to adherens junctions. In contrast, TNF caused marked cell-cell separation, gap formation, and cadherin redistribution in cells co-infected with Ad-lacZ (B). Ad-DNp67 prevented TNF-induced changes (C).