Membrane-bound hemichromes, autologous IgG, and complement C3c fragment; aggregated band 3 and phagocytosis in nonparasitized normal and mutant RBCs. Parameters were measured in nonparasitized normal control (C), alpha-thal trait (A), beta-thal trait (B), HbAS (AS), and HbH (H) RBCs. Hemichromes are expressed in nmol/mL membranes; aggregated band 3, as percentage aggregated band 3 over total band 3; membrane-bound autologous IgG and C3c, as milliabsorbance units/min/107 RBCs; and phagocytosis, as number of phagocytosed RBCs per monocyte. Data are mean values ± SD (vertical bars). Numbers of separate experiments each performed with a different donor were as follows: HbAS, 11; beta-thal trait, 12; alpha-thal trait, 4; and HbH, 5. Significance of differences between nonparasitized normal and mutant RBCs was assessed by t test for paired samples. ^*P < .001; ^**P < .01; and no asterisk, P > .05.