BCR/ABL-induced ROSs cause oxidative DNA damage. 32Dcl3 parental (P) and BCR/ABL-32Dcl3 cells (B/A) were cultured in the presence of IL-3; 2 μM imatinib mesylate, 0.2 μM PDTC, 100 μM DMPO, and 100 μM PBN were added for 48 hours when indicated. (A) Spontaneous DNA damage was detected by the comet assay. (B) Native genomic DNA was run through an ethidium-stained 2.5% agarose gel. (C) Oxidative damage to DNA was probed by EndoIII endonuclease and Fgp glycosylase and was detected by the comet assay. Bars represent the enzyme-dependent increase of DNA damage over that detected in the undigested samples. The error bars represent standard deviation. ^*P < .05 compared with other experimental groups.