IL-2 and IL-4 mRNA quantifications among T cells stimulated by Jk^a synthetic peptides. The sequences of the 4 overlapping peptides tested are indicated at the top of the figure. The Asp280 (D) is indicated in red. The value of cytokine expression after stimulation was calculated after removing the background value of cytokine production by unstimulated peripheral blood mononuclear cells. The star indicates that the value obtained is more than 100. The dotted lines indicate the threshold for specific positive responses due to alloimmunization. Anti-Jk^a alloimmunized donors (d) are in abscissa. The donor DRB1^*/DQB1^* low-resolution typing is indicated at the bottom of the figure.