Figure 5.
Effect of different inhibitors on the peak intracytoplasmic Ca++concentration measured during distinct oscillations elicited by platelet interaction with immobilized VWF under flow. A blood cell suspension, prepared as described in the legend to Figure 2, was perfused for 90 seconds over immobilized VWF at the shear rate of 3000 s–1 and the [Ca++]i of all activated platelets was measured during translocation or stationary adhesion over the next 30 seconds. The bar graphs show the peak values measured during α/β (A) and γ (B) oscillations. The data represent the mean ± 95% confidence intervals of the values measured in 4 different experiments with inhibitors used as described in the legends to Figure 1 and Figure 2. The symbols indicate values that are significantly different from the corresponding controls (*P < .01; ¶ P < .05; Student t test).