Redistribution of adhesion molecules in the DCNK-IS. mDC and NK cell cocultures were stained with either anti-ICAM-1 (A), anti-LFA-1 (B), anti-ICAM-3 (C), anti-DC-SIGN (D), or anti-CD45 mAb (E). Confocal microscopy analyses at 25 minutes highlighted a differential expression of these molecules in DCNK-IS with an exclusion of the ICAM-1 on mDCs and an enrichment of LFA-1 and ICAM-3 on NK cells. DC-SIGN molecules relocate from the mDC cell surface toward the mDC/NK cell synapse (D). CD45 was expressed on the whole NK cell membrane and was not specifically redistributed in the DCNK-IS (E). (A-E) White arrows depict DCNK-IS. (F) Blocking DC-SIGN using neutralizing DC-SIGN mAb decreased the early steps of mDC/NK cell conjugate formation (30% versus 17%; P = .037). Error bars represent SEM.