Comparison of wild-type and Arg134Xaa mutant mRNA stability. (A) Example of DNA and cDNA allele quantifications for one of the transfected cell lines. The percentage above the first T peak refers to the proportion of the mutant allele as determined by the Pyrosequencing software. E indicates time of enzyme addition; S indicates time of substrate addition. (B) Average results of the DNA and cDNA allele quantifications for the 10 transfected cell lines. The units for the x- and y-axes refer to the proportion of mutant alleles. Four replicates for each transfected line were performed. Error bars indicate the standard deviations. The r² value indicates the correlation coefficient between the DNA and cDNA quantifications.