Figure 2.
Figure 2. Visualization of TNF-α– and LPS-induced bladder NF-κB activity. Anesthetized female κB-lacZ mice were challenged intravesically with saline (A-C), LPS (100 μg/mL; D-F), or TNF-α (1 μg/mL; G-I). Twenty-four hours after challenge, bladders were removed and stained with X-gal and photographed as whole mounts (A,D,G-H) or cross-sections (B-C,E-F,I). Tissues were stained with hematoxylin and eosin (B,E) or left unstained (C,F,I). Yellow arrowhead indicates urothelial cells and black arrowhead indicates lymphatic vessels. Cross-sections were visualized with a Nikon compound Eclipse E600 microscope equipped with PlanFluor 40×/0.75 DIC m objective lenses (Nikon). Images were viewed in open air at the following magnifications: A, 300×; B and C, 100×;D,30×; E, F, and I, 400×;G,40×; and H, 60×.

Visualization of TNF-α– and LPS-induced bladder NF-κB activity. Anesthetized female κB-lacZ mice were challenged intravesically with saline (A-C), LPS (100 μg/mL; D-F), or TNF-α (1 μg/mL; G-I). Twenty-four hours after challenge, bladders were removed and stained with X-gal and photographed as whole mounts (A,D,G-H) or cross-sections (B-C,E-F,I). Tissues were stained with hematoxylin and eosin (B,E) or left unstained (C,F,I). Yellow arrowhead indicates urothelial cells and black arrowhead indicates lymphatic vessels. Cross-sections were visualized with a Nikon compound Eclipse E600 microscope equipped with PlanFluor 40×/0.75 DIC m objective lenses (Nikon). Images were viewed in open air at the following magnifications: A, 300×; B and C, 100×;D,30×; E, F, and I, 400×;G,40×; and H, 60×.

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