Multilineage engraftment and β-globin expression in β2m^null NOD-SCID mice. FACS analysis of β2m^null NOD-SCID mouse bone marrow, performed 12 to 16 weeks following transplant with human CD34⁺ cells: β2m Φ (no human cells, row 1), NBM (mock transduced, row 2), TBM (mock transduced, row 3), and TBM-BGI (BGI transduced, row 4). Representative dot-plots showing human CD45 (y-axis, column 1), human CD19 (y-axis, column 2), human CD33 (x-axis, column 2), and human glycophorin A (x-axis, column 3) expression from one mouse in each group. Bone marrow also was labeled intracellularly with HbA Ab, and dot-plots showing HbA expression in the bone marrow of these mice (x-axis, column 4) are shown. All samples in columns 1 to 3 were gated on the basis of appropriate isotype controls, and events falling within the isotype control gate are shown in dark gray. All events labeled positive with the respective human antibodies are shown in red. For intracellular HbA staining, human zeta-globin was used as the negative control, and events falling within this gate are shown in dark gray. HbA-labeled events falling outside this gate are shown in red.