American Society of Hematology

Figure 1.

$Figure 1. RA and TNF induced differentiation of RA-resistant APL cells. Differentiation of cells in response to RA and TNF for varying times. Results are representative of 3 experiments performed in triplicate. (A) Results of NBT reduction assay performed on RA-resistant APL cells treated with RA and TNF for the indicated time periods. Synergy in the number of NBT-positive cells could be observed at concentrations of 10-6 M RA and 10 ng\mL TNF. (B) Cytofluorimetric analysis of surface marker expression. Percentage of cells expressing the monocyte-specific CD14 and myeloid-specific CD11b cell surface markers of differentiation was determined by using monoclonal fluorescein isothiocyanate (FITC; CD14) and phycoerythrin (PE; CD11b) labeled antibodies. Results are representative of 1 experiment of 3 performed in triplicate.$

RA and TNF induced differentiation of RA-resistant APL cells. Differentiation of cells in response to RA and TNF for varying times. Results are representative of 3 experiments performed in triplicate. (A) Results of NBT reduction assay performed on RA-resistant APL cells treated with RA and TNF for the indicated time periods. Synergy in the number of NBT-positive cells could be observed at concentrations of 10^-6 M RA and 10 ng\mL TNF. (B) Cytofluorimetric analysis of surface marker expression. Percentage of cells expressing the monocyte-specific CD14 and myeloid-specific CD11b cell surface markers of differentiation was determined by using monoclonal fluorescein isothiocyanate (FITC; CD14) and phycoerythrin (PE; CD11b) labeled antibodies. Results are representative of 1 experiment of 3 performed in triplicate.

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