Reciprocal effects of RelB and p100ΔN on the differentiation of CD11b⁺ DCs. CD34⁺ cells were transduced with retroviral vectors encoding p100ΔN, empty control, RelB, or IκBα-SR upstream of IRES-GFP and were induced to develop into DCs. (A) FACS diagrams show transduced cells (gated on GFP⁺ cells, as shown in Figure 1A) analyzed for CD11b versus CD14 (day 8) or CD11b versus CD1a (days 8, 12). Markers were set according to negative control stainings. Data are representative of 4 independent experiments. Numbers indicate percentages of cells within the quadrants. (B) CD14 MFI (numbers to the right of each bar) of cells from FACS diagrams shown in panel A. ▪ indicates CD14⁺CD11b⁺ cells (top right quadrants). ▦ indicates CD14⁺CD11b^- cells (top left quadrants). (C) Bar diagrams represent mean ± SD of CD14⁺CD11b⁺ (day 8; left) or CD1a⁺CD11b⁺ (day 12; right) cells in 4 independent experiments. Paired Student t test statistical analysis: percentage CD14⁺CD11b⁺ (left bar diagrams; p100ΔN vs control, P = .002; RelB vs control, P = .004); percentage CD1a⁺CD11b⁺ (right bar diagrams; p100ΔN vs control, P = .046; RelB vs control, P = .003).