Comparison of p100ΔN and IκBα-SR. CD34⁺ cells were transduced with retroviral vectors encoding the indicated molecules and were induced to develop into DCs. (A) Histograms represent expression patterns of the indicated cytoadhesion molecules by gated GFP⁺ cells. (B) Microphotographs show culture morphology of cells after 30-minute 1g sedimentation over 7.5% HAS. (left) Control (empty vector). (right) IκBα-SR. (C) U937 NF-κB–GFP reporter cells were infected with IκBα-SR–IRES-mCD8α, p100ΔN–IRES-mCD8α, or empty vector (control). Two days after infection, cells were stimulated with TNF-α (25 ng/mL) for 48 hours and were analyzed for GFP induction by FACS. Overlay histograms show control-transduced cells with or without TNF-α stimulation, and IκBα-SR or p100ΔN-transduced cells stimulated with TNF-α. (D) Annexin versus 7-AAD stainings of representative day 8–generated GFP⁺-gated cells. Numbers indicate percentages of gated cells within the quadrants. (E) Bar diagrams show consistent time-linked decreases in average percentages of GFP⁺ cells in IκBα-SR compared with p100ΔN-, control-, or RelB-transduced cultures. Bars represent mean ± SD percentage decreases of GFP⁺ cells determined at days 7 to 9 after gene transduction over values observed at 48 to 72 hours after gene transduction; 48- to 72-hour values represent 100%. FACS data in panels D and E are representative of 3 independent experiments. Bar histograms represent the mean ± SD of 4 independent experiments. P = .009 for IκBα-SR compared with control.