RelB inhibition impairs the generation of monocytes but not of LCs. Gene-transduced progenitor cells were stimulated in the presence of 0.5 ng/mL TGF-β1 to generate LCs. (A) CD1a⁺GFP⁺ and CD1a^-GFP⁺ cells were gated as shown (left diagram) and analyzed for Langerin versus CD11b expression. (B) Gated GFP⁺ cells analyzed for E-cadherin versus CD1a expression. Region settings were performed according to control stainings. Data are representative of at least 3 independent experiments. Percentages on the plots are the frequency of gated cells lying within the indicated regions.