Figure 2.
Figure 2. Identification of the rp gene defect. (A) Fine-linkage map of the rp critical interval on Chr 7. (B) A C>T transition creates a premature stop codon in the rp gene. (C) A 672-bp fragment within exon 2 was amplified from gDNA using forward primer 5′-AATGCTACCACGCATTGGAGGCCT-3′ and reverse primer GCCATCTCGTGT-GACCTAGTTCTA-3′ and digested with Fnu4HI. The rp mutation removes an Fnu4HI site, changing 63- and 71-bp fragments to a 134-bp fragment that was used to genotype mice.

Identification of therpgene defect. (A) Fine-linkage map of the rp critical interval on Chr 7. (B) A C>T transition creates a premature stop codon in the rp gene. (C) A 672-bp fragment within exon 2 was amplified from gDNA using forward primer 5′-AATGCTACCACGCATTGGAGGCCT-3′ and reverse primer GCCATCTCGTGT-GACCTAGTTCTA-3′ and digested with Fnu4HI. The rp mutation removes an Fnu4HI site, changing 63- and 71-bp fragments to a 134-bp fragment that was used to genotype mice.

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