Immunoblot analysis of Rac1 and Rac2 levels in the mouse peritoneal neutrophils. Representative immunoblot of Rac1 and Rac2 expression in peritoneal neutrophils. Neutrophils were recruited using an intraperitoneal injection of sodium periodate. After 4 hours a peritoneal lavage was performed to collect neutrophils. As expected from genotyping, we were able to generate mice with neutrophils lacking Rac1 expression (Rac1 null), Rac2 expression (Rac2 null), and both Rac1 and Rac2 expression (Rac1/2 null). Antibody against β-actin was used as a loading control.