Leukocyte rolling flux and velocity in cremaster microcirculation of C57Bl/6 mice treated with TNF-α. Leukocyte rolling flux (A) and leukocyte rolling velocity (B) in cremaster microcirculation of C57Bl/6 mice treated 3 hours before with TNF-α applied locally (0.5 μg/mouse). The effects of anti–P-selectin, anti–E-selectin, or anti–PSGL-1 alone or in combination were tested. ▪ represents the values for leukocyte rolling and leukocyte rolling velocity under control conditions. Data are presented as mean ± SEM. ^*P < .05 relative to C57Bl/6 mice treated with TNF-α (second column). N/D indicates not determined.