Leukocyte rolling flux and velocity in cremaster microcirculation of P-selectin^-/- mice treated with TNF-α. Leukocyte rolling flux (A) and leukocyte rolling velocity (B) in cremaster microcirculation of P-selectin^-/- mice treated 3 hours before with TNF-α applied locally (0.5 μg/mouse). The effects of anti–PSGL-1 antibody (4RA10 or 2PH1) and anti–E-selectin antibody were tested. Data are presented as mean ± SEM. ^*P < .05 relative to untreated value; ^**P < .05 relative to 4RA10 treatment.