Leukocyte rolling flux in cremaster microcirculation of C57Bl/6 and PSGL-1^-/- mice treated 3 hours before with TNF-α intraperitoneally or locally (0.5 μg/mouse). The effect of systemic or local injection of TNF-α is shown in C57Bl/6 and PSGL-1^-/- mice (A). Also, the effect of PSGL-1 antibody (4RA10) was tested in C57Bl/6 mice injected systemically with TNF-α (B). Data are presented as mean ± SEM. ^*P < .05 relative to PSGL-1^-/- mice that received local injection of TNF-α.