TCR spectratyping reveals the presence of a diverse TCR repertoire following depletion of alloreactive or CMV-specific T cells. (A) Molecular diversity of CD4^hiCD38⁺ T cells. Purified CD4^hiCD38⁺ T cells obtained following CMV stimulation demonstrated more skewing from a diverse Gaussian pattern relative to the CD4^hiCD38⁺ T-cell subset obtained after allogeneic stimulation. (B) Diverse repertoire of residual CD4^intCD38^– T cells following depletion of reactive CD4^hiCD38⁺ T cells. The TCR repertoire of CD4^intCD38^– T cells, obtained after either CMV or allogeneic stimulation, was extremely diverse, as evidenced by a normal Gaussian TCR spectratype. In each example, representative TCR Vβ subsets from 12 of 23 assessed TCR Vβ subsets. Results are from a single donor and representative of 2 similar experiments.