Figure 4.
Effect of antibodies and peptides on cell adhesion. Confluent ECs plated on gelatin-coated coverslips were incubated in McCoy 5A medium (A), with 5 μg/mL 7E3 (B), 1 mM GRGDS (C), or 5 μg/mL α5β1 antibody (D). After 24 hours, the cells were fixed, washed with PBS twice, stained with propidium iodide, and the coverslips were viewed under a fluorescence microscope.