Figure 3.
![Figure 3. RFC gene expression and electrophoretic mobility shift assay in parental cells and their MTX-resistant sublines. Semiquantitative RT-PCR analysis of the RFC gene (A) along with a GAPDH control was performed as previously described.25,26,29 Electrophoretic mobility shift assay (B) was performed by the binding of nuclear proteins to their [32P]-labeled consensus double-stranded oligonucleotides followed by visualization with a Phosphorimager as detailed elsewhere.25,26](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/13/10.1182_blood-2004-04-1493/6/zh80240471130003.jpeg?Expires=1742847046&Signature=GtLUbedNsMlamOmR6cigXJTxDCkYpH2B0vsXuRMqTjVsi15mGOJsI5~zj22BlVY5lylbX5HzT3lTv0PY0MBBl3VeUybAMyvPPwzwtLYYVlmsa-3lhHek6R9eHvyNLvddO-JdWFapLWLxqzwJEnOh2r2MWZjqMO3Cwtv~rcwAxcEUfp-GnMIZspXLrc39pPizCqHNwUNlFFHMYdeLfPU3bk9qvPUayLbeFE~NyZ8aN7CgS1txc9tiobfW2nUpzshLhVI8G1BQuvl8oyZcx1YCYKriKqM15gmSE0q-UiiJbyoPntH2aSKUXf94ehxc9nm3VLc540BMXPs48yt2EDYQ0A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
RFC gene expression and electrophoretic mobility shift assay in parental cells and their MTX-resistant sublines. Semiquantitative RT-PCR analysis of the RFC gene (A) along with a GAPDH control was performed as previously described.25,26,29 Electrophoretic mobility shift assay (B) was performed by the binding of nuclear proteins to their [32P]-labeled consensus double-stranded oligonucleotides followed by visualization with a Phosphorimager as detailed elsewhere.25,26
