Reduction of GCAD by Bcl-2 overexpression 30 days after transplantation. Representative sections of cardiac allografts stained with elastic van Gieson for evaluation of GCAD in the (Ai) WT donor heart group and (Aii) the Bcl-2 Tg donor heart group. (Ai) Note that marked fibrointimal thickening and luminal narrowing, morphologically resembling typical human GCAD, are observed in the WT donor heart group. (Aii) In contrast, decreased intimal thickening and preserved vessel lumen are observed in the Bcl-2 Tg donor heart group. A Leica DMRB fluorescent microscope (Leica Microsystems, Frankfurt, Germany) and SPOT Advanced Version 3.4.2 software (Diagnostic Instruments, Sterling Heights, MI) were used. Original magnification, × 400. (B) Morphometric assessment of cardiac grafts. Note that compared with parameters in the WT donor heart group, all 3 GCAD parameters are significantly lower in the Bcl-2 Tg donor heart group 30 days after transplantation. ▪ indicates the data from WT heart grafts (n = 8); □, those from the Bcl-2 Tg heart grafts (n = 8). Mean ± SD values are shown.