Effects of SDF-1 on differentiation of c-kit⁺/lin^– cells onto FN. (A) Negative selection for lineage-negative cells was performed prior to isolating c-kit⁺ cells from mouse BM mononuclear cells. FACS analysis showed that lineage marker selection allowed recovery of cells positive for c-kit, CD34, and Sca-1 markers as shown by histogram overlay of each specific antibody over isotype control antibody. (B) C-kit⁺/lin^– cells were plated into glass chamber slides coated with FN in the presence or the absence of 100 ng/mL SDF-1. Cells taking up Ac-LDL-DiI were counted as in Figure 1. *P < .05; n = 3. Error bars indicate standard errors. C indicates control-untreated cells.