Effects of various differentiation-inducing agents on PLSCR1 expression in leukemic cell lines and Cos-7 cells. (A-B) NB4 and U937 cells were treated with the differentiation inducers 10^-6 M ATRA, 1% DMSO, 100 nM PMA, 2 mM SB, or 2.5 × 10^-7 M VD3 for 3 days; then real-time PCR (A) and Western blots (B) for PLSCR1 with β-actin as loading control were performed as described in “Materials and methods.” (C) HL60 cells were treated with 1% DMSO and/or 1 μM rottlerin for 24 hours, and PLSCR1 was detected by Western blot with β-actin as loading control. (D) Cos-7 cells were treated with ATRA (10^-6 M) or PMA (200 nM and 500 nM) for day(s) shown, and PLSCR1 was detected by Western blot with β-actin as loading control. All experiments were repeated at least 3 times with similar results.